New Botany Study Results Reported from Molecular Biology Laboratory (Analysis of variation for apomictic reproduction in diploid Paspalum rufum)

Life Science Weekly |

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Life Science Research. According to news reporting out of Santa Fe, Argentina, by NewsRx editors, research stated, "The diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours." Our news journalists obtained a quote from the research from Molecular Biology Laboratory, "Samples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. In open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes. The results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level." According to the news editors, the research concluded: "Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment." For more information on this research see: Analysis of variation for apomictic reproduction in diploid Paspalum rufum. Annals of Botany, 2014;113(7):1211-1218. Annals of Botany can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press -; Annals of Botany - Our news journalists report that additional information may be obtained by contacting L. Delgado, UNR, Mol Biol Lab, Fac Ciencias Agr, Zavalla, Santa Fe, Argentina. Additional authors for this research include F. Galdeano, M.E. Sartor, C.L. Quarin, F. Espinoza and J.P.A. Ortiz (see also Life Science Research). Keywords for this news article include: Santa Fe, Argentina, South America, Life Science Research Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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