New Botany Study Findings Recently Were Reported by Y.F. Huang and Co-Researchers (A negative MYB regulator of proanthocyanidin accumulation,...

Life Science Weekly |

New Botany Study Findings Recently Were Reported by Y.F. Huang and Co-Researchers (A negative MYB regulator of proanthocyanidin accumulation, identified through expression quantitative locus mapping in the grape berry) By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Life Science Research have been published. According to news reporting out of Montpellier, France, by NewsRx editors, research stated, "Flavonoids are secondary metabolites with multiple functions. In grape (Vitis vinifera), the most abundant flavonoids are proanthocyanidins (PAs), major quality determinants for fruit and wine." Our news journalists obtained a quote from the research, "However, knowledge about the regulation of PA composition is sparse. Thus, we aimed to identify novel genomic regions involved in this mechanism. Expression quantitative trait locus (eQTL) mapping was performed on the transcript abundance of five downstream PA synthesis genes (dihydroflavonol reductase (VvDFR), leucoanthocyanidin dioxygenase (VvLDOX), leucoanthocyanidin reductase (VvLAR1), VvLAR2 and anthocyanidin reductase (VvANR)) measured by real-time quantitative PCR on a pseudo F1 population in two growing seasons. Twenty-one eQTLs were identified; 17 of them did not overlap with known candidate transcription factors or cis-regulatory sequences. These novel loci and the presence of digenic epistasis support the previous hypothesis of a polygenic regulatory mechanism for PA biosynthesis. In a genomic region co-locating eQTLs for VvDFR, VvLDOX and VvLAR1, gene annotation and a transcriptomic survey suggested that VvMYBC2-L1, a gene coding for an R2R3-MYB protein, is involved in regulating PA synthesis. Phylogenetic analysis showed its high similarity to characterized negative MYB factors. Its spatiotemporal expression profile in grape coincided with PA synthesis." According to the news editors, the research concluded: "Its functional characterization via overexpression in grapevine hairy roots demonstrated its ability to reduce the amount of PA and to down-regulate expression of PA genes." For more information on this research see: A negative MYB regulator of proanthocyanidin accumulation, identified through expression quantitative locus mapping in the grape berry. New Phytologist, 2014;201(3):795-809. New Phytologist can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; New Phytologist - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137) Our news journalists report that additional information may be obtained by contacting Y.F. Huang, SupAgro, UMR1334, AGAP, F-34060 Montpellier, France. Additional authors for this research include S. Vialet, J.L. Guiraud, L. Torregrosa, Y. Bertrand, V. Cheynier, P. This and N. Terrier (see also Life Science Research). Keywords for this news article include: France, Europe, Reductase, Montpellier, Enzymes and Coenzymes, Life Science Research Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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