Investigators from University of Turku Report New Data on Science (Peptic Fluorescent “signal-on” and “signal-off” Sensors Utilized for the Detection Protein Post-translational Modifications)

NewsRx Life Science Daily |

2019 APR 15 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- A new study on Science is now available. According to news reporting originating from Turku, Finland, by NewsRx correspondents, research stated, “Protein post-translational modifications (PTMs) are typically enzyme-catalyzed events generating functional diversification of proteome; thus, multiple PTM enzymes have been validated as potential drug targets. We have previously introduced energy-transfer-based signal-modulation method called quenching resonance energy transfer (QRET), and utilize it to monitor PTM addition or removal using the developed peptide-break technology.”

Financial support for this research came from Academy of Finland.

Our news editors obtained a quote from the research from the University of Turku, “Now we have reinvented the QRET technology, and as a model, we introduced the tunable fluorescent ‘signal-on’ and ‘signal-off’ detection scheme in the peptide-break PTM detection. Taking the advantage of time-resolved fluorescence-based single-label detection technology, we were able to select the signal direction upon PTM addition or removal by simply introducing different soluble Eu3+-signal-modulating molecule. This enables the selection of positive signal change upon measurable event, without any additional labeling steps, changes in assay condition or Eu3+-reporter. The concept functionality was demonstrated with four Eu3+-signal modulators in a high-throughput compatible kinase and phosphatase assays using signal-on and signal-off readout at 615 nm or time-resolved Forster resonance energy transfer at 665 nm.”

According to the news editors, the research concluded: “Our data suggest that the introduced signal modulation methodology provides a transitional fluorescence-based single-label detection concept not limited only to PTM detection.”

For more information on this research see: Peptic Fluorescent “signal-on” and “signal-off” Sensors Utilized for the Detection Protein Post-translational Modifications. ACS Omega, 2019;4(2):4269-4275. ACS Omega can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA.

The news editors report that additional information may be obtained by contacting K. Kopra, University of Turku, Dept. of Chemistry, Mat Chem & Chem Anal, Vatselankatu 2, Sf-20500 Turku, Finland. Additional authors for this research include V. Eskonen, T. Seppala, J. Jakovleva, H. Harma and R. Huttunen.

The direct object identifier (DOI) for that additional information is: https://doi.org/10.1021/acsomega.8b03672. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.

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